Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy.
نویسندگان
چکیده
The tongue consists of a complex, multiscale array of myofibers that comprise the anatomical underpinning of lingual mechanical function. 3-D myoarchitecture was imaged in mouse tongues with diffusion spectrum magnetic resonance imaging (DSI) at 9.4 T (b(max) 7000 smm, 150-microm isotropic voxels), a method that derives the preferential diffusion of water/voxel, and high-throughput (10 fps) two-photon microscope (TPM). Net fiber alignment was represented for each method in terms of the local maxima of an orientational distribution function (ODF) derived from the local diffusion (DSI) and 3-D structural autocorrelation (TPM), respectively. Mesoscale myofiber tracts were generated by alignment of the principal orientation vectors of the ODFs. These data revealed a consistent relationship between the properties of the respective ODFs and the virtual superimposition of the distributed mesoscale myofiber tracts. The identification of a mesoscale anatomical construct, which specifically links the microscopic and macroscopic spatial scales, provides a method for relating the orientation and distribution of cells and subcellular components with overall tissue morphology, thus contributing to the development of multiscale methods for mechanical analysis.
منابع مشابه
ultiscale structural analysis of mouse lingual yoarchitecture employing diffusion spectrum magnetic esonance imaging and multiphoton microscopy
erry A. Gaige yuk Sang Kwon uangping Dai ictor C. Cabral uopeng Wang oon Sung Nam evin P. Engelward an J Wedeen eter T. C. So ichard J. Gilbert assachusetts Institute of Technology epartment of Mechanical and Biological Engineering ambridge, Massachusetts 02139 and assachusetts General Hospital thinoula A. Martinos Center for Biomedical Imaging harlestown, Massachusetts 02129 Abstract. The tong...
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عنوان ژورنال:
- Journal of biomedical optics
دوره 13 6 شماره
صفحات -
تاریخ انتشار 2008